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fractalkine specific sirna  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology fractalkine specific sirna
Fig. 3. Immunoblot analysis of fractalkine protein expression in HUVECs after silencing NF-κB p65. HUVECs were transfected with NF-κB p65 <t>siRNA</t> or control siRNA (Cont siRNA) and treated with TNF-α with or without EGCG (50 µmol/L) for 6 h. Actin was used as the loading control. Densitometric analyses are presented as the relative ratio of fractalkine to actin. The relative ratio compared to the control is arbitrarily defined as 1. Note that silencing NF-κB p65 reduced TNF-α-induced fractalkine protein expression. Bars represent the mean±SD from 3 different experiments. *, p<0.05 vs. Cont siRNA only; #, p<0.05 vs. TNF-α plus Cont siRNA.
Fractalkine Specific Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3. Immunoblot analysis of fractalkine protein expression in HUVECs after silencing NF-κB p65. HUVECs were transfected with NF-κB p65 siRNA or control siRNA (Cont siRNA) and treated with TNF-α with or without EGCG (50 µmol/L) for 6 h. Actin was used as the loading control. Densitometric analyses are presented as the relative ratio of fractalkine to actin. The relative ratio compared to the control is arbitrarily defined as 1. Note that silencing NF-κB p65 reduced TNF-α-induced fractalkine protein expression. Bars represent the mean±SD from 3 different experiments. *, p<0.05 vs. Cont siRNA only; #, p<0.05 vs. TNF-α plus Cont siRNA.

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: Epigallocatechin-3-O-gallate decreases tumor necrosis factor-alpha-induced fractalkine expression in endothelial cells by suppressing NF-kappaB.

doi: 10.1159/000257494

Figure Lengend Snippet: Fig. 3. Immunoblot analysis of fractalkine protein expression in HUVECs after silencing NF-κB p65. HUVECs were transfected with NF-κB p65 siRNA or control siRNA (Cont siRNA) and treated with TNF-α with or without EGCG (50 µmol/L) for 6 h. Actin was used as the loading control. Densitometric analyses are presented as the relative ratio of fractalkine to actin. The relative ratio compared to the control is arbitrarily defined as 1. Note that silencing NF-κB p65 reduced TNF-α-induced fractalkine protein expression. Bars represent the mean±SD from 3 different experiments. *, p<0.05 vs. Cont siRNA only; #, p<0.05 vs. TNF-α plus Cont siRNA.

Article Snippet: Fractalkine-specific siRNA (Santa Cruz Biotechnology, Santa Cruz, CA, USA), NF-κB p65 siRNA (human specific) (Cell Signaling Technology), negative control siRNA (Dharmacon Research, Lafayette, CO, USA) were used.

Techniques: Western Blot, Expressing, Transfection, Control

Fig. 6. EGCG reduces adhesion of monocytes to HUVEC monolayers. (A) Representative images of the adhesion of monocytes to HUVEC monolayers. HUVECs were incubated with the indicated doses of EGCG, fractalkine-specific siRNA, control siRNA, antibody against fractalkine, control antibody (Cont antibody) and TNF-α (10 ng/mL) for 4 h. Fluorescence-labelled monocytes were added to the HUVEC monolayer and allowed to adhere for 2 h. The adhered cells were measured as described in Materials and Methods. (B) Quantification of monocyte adhesion to HUVECs. Data are from three experiments and are expressed as a percentage of adhesion to TNF-α-induced HUVECs. Bars represent the mean±SD from three experiments. *, p<0.05 vs. control (CB); #, p<0.05 vs. TNF-α.

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: Epigallocatechin-3-O-gallate decreases tumor necrosis factor-alpha-induced fractalkine expression in endothelial cells by suppressing NF-kappaB.

doi: 10.1159/000257494

Figure Lengend Snippet: Fig. 6. EGCG reduces adhesion of monocytes to HUVEC monolayers. (A) Representative images of the adhesion of monocytes to HUVEC monolayers. HUVECs were incubated with the indicated doses of EGCG, fractalkine-specific siRNA, control siRNA, antibody against fractalkine, control antibody (Cont antibody) and TNF-α (10 ng/mL) for 4 h. Fluorescence-labelled monocytes were added to the HUVEC monolayer and allowed to adhere for 2 h. The adhered cells were measured as described in Materials and Methods. (B) Quantification of monocyte adhesion to HUVECs. Data are from three experiments and are expressed as a percentage of adhesion to TNF-α-induced HUVECs. Bars represent the mean±SD from three experiments. *, p<0.05 vs. control (CB); #, p<0.05 vs. TNF-α.

Article Snippet: Fractalkine-specific siRNA (Santa Cruz Biotechnology, Santa Cruz, CA, USA), NF-κB p65 siRNA (human specific) (Cell Signaling Technology), negative control siRNA (Dharmacon Research, Lafayette, CO, USA) were used.

Techniques: Incubation, Control, Fluorescence